DESCRIPTION

Part of the ZymoPURE™ Plasmid Kits collection, the ZymoPURE™ Plasmid Miniprep Kit features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE™ Plasmid Miniprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

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TECHNICAL SPECIFICATIONS

Applicable For	Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
Binding Capacity	100 µg
Culture Input	≤ 5 mL
Elution Volume	25 µl
Endotoxin Levels	≤ 1 EU/µg
Equipment	Microcentrifuge and/or vacuum manifold
Format	Spin Column
Processing Time	15 min
Purity	Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Recommended Sample Volume	≤ 5.0 ml
Size Range	Up to ^~200 kb
Yield	Up to 100 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized.
Supplemental Info	How to Increase Plasmid Yield DOs and DON’Ts of Plasmid Purification